The scan(s) and data are available for unlimited academic/research purposes. Please cite the web page as follows: O.W. Henson, et al., Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill and The Center for In Vivo Microscopy, Duke University, Durham, NC, Copyright 2000.

The scans are available as self extracting archives. Double click on the file and it will expand into an uncompressed file in TIFF or PICS format. Tecnical information such as field of view and dimension can be found in the headfile which is in the "Info folder" with each stack. Headfiles are text files and can be opened with Word or SimpleText. Not all scans have headfiles. Additional descriptions of the scans are found on the Additional information web page.

Comments and questions can be directed to Dr. Henson (

Segmented Stacks

Segmented stacks have structures already designated. Not all stacks have all structures segmented. The following color scheme was used for 3D reconstructions. Each number shown below represents a shade of gray in NIH Image. Reconstructed structures in NIH Image were changed to these values to facilitate analysis of individual structures, especially when being transfered to another platform (i.e. Silicon Graphics).

Color - structure

0 Otolyths
1 Primary Osseus Spiral Laminae
2 Tympanic Bone
5 Otic Capsule
7 Stapedial Bone (Skeletal Element of Paauw) sometimes Tympanic Bone
10 Spiral Ligament
20 Incus/Malleus
30 Stapes
40 Scala Tympani
45 Cochlear aquiduct
60 Scala Media
70 Semi-Circular Canals and Vestibule
80 Scala Vestibuli
90 Stapedial Muscle
100 Tympanic Mmebrane
110 Endolymphatic Sac
120 Cochlea Nerve
125 Stapedial Nerve
130 Stapedial artery
135 Round Window Membrane
140 Stapedial Muscle or tensor tympani

These colors can be changed using the Change color macro in NIH Image. See the NIH Image Segmenting web page for more information.